Types of Cell Culture Media- Cell culture media is a complex mix of components used in the growth and maintenance of cultured cells. This article covers DMEM, IMDM, RPMI, and RPMI-H. You can also learn about other media types, including a serum-free alternative. Listed below are some differences between these media types.
Table of Contents
DMEM is cell culture media used for the cultivation of cells in culture dishes. It is composed of a mixture of water and glucose that is enriched with amino acids and vitamins. It also contains inorganic salts like calcium and magnesium. This is an ideal mixture for the culture of various types of cells. However, it does need to be supplemented to maintain optimal conditions.
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Purpose of DMEM-Culture Media
The optimal medium for cell culture depends on the purpose of the experiment. For example, if you need to generate large numbers of cells quickly, you will need to choose a medium that supports maximal growth of cells while maintaining their viability at high cell densities. In addition, if you’re producing viruses, you’ll need a medium that can sustain high densities of cells without compromising viability. DMEM High Glucose (H-21) is the preferred cell culture medium for this purpose.
Importance of DMEM-Culture Media
Exposure to fluorescent light can negatively affect cell growth. Exposed DMEM results in an average of 10 to 40% reduction in cell yield compared to non-exposed control medium. In addition, DMEM exposed to light is prone to photoactivation of riboflavin, which leads to the production of tryptophan and peroxide. Exposure to light decreased cell yields in a dose-dependent manner; the higher the light intensity, the greater the decline in cell growth capacity.
The pH of culture medium is very important for cell growth. In many media formulations, pH indicators such as phenol red are used to monitor the condition. Phenol red has estrogen-like properties, so it’s usually excluded from cell types that express the estrogen receptor. However, media without phenol red are available for colorimetric assays. The presence of phenol red interferes with spectrophotometric readouts, so it’s better to opt for media without this additive.
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In live-cell fluorescence imaging, researchers must be able to visualize weak fluorescent signals against a background of high-intensity light. Because most cell culture media contain components that emit auto fluorescence when excited, they negatively affect fluorophore signal-to-noise ratios.
IMDM is a type of culture medium that contains a high concentration of nutrients. It is designed to promote cell growth while remaining within the physiological limits of cells. IMDM is useful for culturing many different species. IMDM contains several essential ingredients for culture, including amino acids, vitamins, inorganic salts, glucose, and serum, which contains growth and attachment factors. In addition, it helps maintain the pH and osmolality of the culture.
Purpose of IMDM- Culture Media
IMDM is a modified version of DMEM, a commonly used cell culture medium. It contains additional amino acids and vitamins, as well as selenium. IMDM is also suited for rapid cell proliferation in high density cultures. It is available in powdered form. It can be used in the manufacture of animal models and for regenerative medicine.
The IMDM cell culture medium is especially useful for high-density cell cultures, as it provides serum-free conditions. The media is also useful for supporting B and T lymphocytes, two cell types that have a high proliferative capacity. IMDM also allows for the culture of other cell types under serum-free conditions.
Application of IMDM- Culture Media
For the production of recombinant proteins, it is necessary to maintain high cell density. However, the growth of these cells may compete with the nutrients necessary for protein production. This is why it is important to determine the density required for the desired productivity. It is also important to remember that any changes in the medium must not adversely affect the quality of the resulting product.
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The IMDM cell culture media is prepared by adding several nutrients. These include organic and inorganic compounds, salts, O2, and CO2 gas phases, and serum proteins, carbohydrates, and cofactors. Each of these ingredients has a specific function. Some serve immediate survival, while others are used for prolonged growth and specialized functions.
The main ingredients in IMDM cell culture medium are sodium bicarbonate, L-glutamine, and glucose. It contains approximately four mM L-glutamine and 1500 mg of sodium bicarbonate. It is safe to use in high-density cell cultures, but it may not be suitable for cultures of estrogen-sensitive cells.
RPMI- Culture Media
RPMI is a general purpose enriched medium that was developed in 1966 by John Moore at the Roswell Park Memorial Institute. It was originally developed to grow human leukemia cells in monolayer and suspension cultures. Today, RPMI is a standard cell culture medium, used to culture various mammalian cells.
Purpose of RPMI – Culture Media
This medium is used for a variety of cell cultures, including stem cells, keratinocytes, and neurons. It is available from several major suppliers, including Invitrogen. RPMI is also used for organogenesis and B-type lamins in mice. RPMI also contains sodium bicarbonate, which is important for maintaining the proper pH.
Most cell culture media are composed of nutrient-rich solution to maintain the growth of cells in a culture dish. The main ingredients of culture media include amino acids, inorganic salts, glucose, and serum. The serum serves as a source of growth factors, attachment factors, and hormones. In addition, it helps maintain the pH and osmolality levels of the cells in the culture dish.
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The pH level of a culture medium is important for the success of the experiment. Using a pH indicator in a culture medium allows the researcher to monitor the pH level continuously. It also changes color as metabolites released by the cells change its pH levels. At low pH, it turns yellow, while at high pH, it turns purple. pH 7.4 is bright red. However, phenol red can mimic the action of certain steroid hormones and estrogens, and therefore should not be used when working with cells that are sensitive to estrogen.
Application of RPMI- Culture Media
The type of cell culture medium used in a study can affect the outcome of the experiment. The choice of medium depends on the types of cells that are to be cultured, the purpose of the experiment, and the resources available in the laboratory. The media must be appropriate for the type of cells being cultured, because different cell types need different conditions to grow. In addition to the type of culture medium used, the optimum growth conditions of each cell type must be established experimentally to ensure the best results.
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The amino acids found in cell culture media are important for the growth of the cells. They are the building blocks of proteins, and they must be present in the culture medium. Cells cannot synthesize these essential amino acids, so their addition to the culture medium is necessary to encourage cell growth. In addition, these amino acids determine the maximum cell density possible.
L-glutamine is especially important in the cell culture medium. It is a nitrogen-rich amino acid, and it is an important secondary energy source for metabolic processes. However, L-glutamine is an unstable amino acid, so it should be added before use.
RPMI-H is an essential component of most cell culture cultures. Depending on the type of cells, this medium is used to grow a variety of cells. This culture medium contains a range of nutrients that will support the growth of the cells. For most cell lines, the appropriate temperature is 37degC with 5% CO2. Before initiating the culture, remove any ice crystals from the cell culture flask by gently centrifugation. Once the cells have reached a suspension state, remove the flask and store the supernatant at 4degC for later use.
Purpose of RPMI-H – Culture Media
RPMI-H cell culture media contains phosphate, a mineral that is essential for cell growth. The concentration of phosphate in RPMI-H is high enough to stimulate cellular proliferation without causing a metabolic burden on cells. As a result, the high concentration of phosphate in this medium allows for the cultivation of a wide range of cell types.
Sodium bicarbonate is an important component of most cell culture media. It is important to remember that this is a natural compound that occurs in the human body. Because of this, it mimics the physiology of the body and minimizes the potential for toxic side effects. The pH indicator Phenol Red is also included in the cell culture media. The medium will turn a yellow hue when pH is too low, a purple hue if it is too high, and an orangey-red hue means that the pH is at the proper physiological level.
Application of RPMI-H – Culture Media
RPMI-H cell culture media is a useful tool for scientists who want to study the behavior of cell cultures. It contains a range of nutrients that can help researchers study the biological processes that affect the body. In addition, it can be used to develop biopharmaceuticals.
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