Created by Witfire- Scientific Community Annealing Temperature Calculator Annealing Temperature Calculator Annealing temperature calculator in molecular biology is the temperature at which complementary strands of DNA bind to form a double-stranded molecule. The annealing temperature depends on several factors, including the length of the primers and the percentage of GC content in the DNA sequence. A common formula used to calculate the annealing temperature is: … Continue reading Annealing Temperature Calculator