Category: Microbiology

Michaelis Menten Kinetics
Biochemistry

Michaelis Menten Kinetics – Mechanism and Applications

Michaelis Menten kinetics is a model used to describe the rate of enzyme-catalyzed reactions. The model was developed by Leonor Michaelis and Maud Menten in 1913 and is still used today to explain how enzymes work. The model is based on enzymes binding to substrates to form an enzyme-substrate complex. The substrate then undergoes a chemical reaction, releasing the products from the complex. The Michaelis …

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Dr. Anubhav Sharma
Microbiology

Different types of Staining Methods in Cell Biology?

Types of Staining Methods in Cell biology- Biological staining enhances contrast in samples, making different structures more visible. Many types of staining methods are used in cell biology, each with advantages and disadvantages. This article will look closely at some of the most popular staining methods and how they’re used to study cells. Table of Content What is cell staining? Cell staining is a technique … Continue reading Different types of Staining Methods in Cell Biology?

Dr. Anubhav Sharma
Folin Lowry Method
Biochemistry

Principle, Procedure and Results of Folin Lowry Method

Principle, Procedure and Results of Folin Lowry Method- One of the essential methods for estimating protein is the Folin Lowry method. Folin and Lowry developed it in 1915. This method is based on the principle that all proteins yield the same amount of nitrogen when oxidized. Principle of Folin Lowry method This method is a colorimetric assay for determining nitrogen in organic compounds. The method … Continue reading Principle, Procedure and Results of Folin Lowry Method

Dr. Anubhav Sharma
Chromosome Staining Techniques
Microbiology

Chromosome Staining Techniques: Which one is The Best?

Chromosome Staining Techniques: There are many different ways to view chromosomes and studying them can give important information about genetic disorders. In this article, we will compare and contrast three popular staining techniques – G-banding, C-banding, and Q-banding. Table of Content What is chromosome staining? Chromosome staining is a technique used to visualize chromosomes and their features under the microscope. There are several different methods … Continue reading Chromosome Staining Techniques: Which one is The Best?

Dr. Anubhav Sharma
coronavirus statistics on screen
Microbiology

What is Pathogenesis? The Development of a Disease?

Pathogenesis is the medical term for the development of a disease. It encompasses all the events that lead up to the manifestation of symptoms. This includes the initial exposure to a trigger, the incubation period, the progression of symptoms, and ultimately the organism’s death. What is Pathogenesis? Pathogenesis is the development of a disease. It is the process by which a disease develops and progresses. … Continue reading What is Pathogenesis? The Development of a Disease?

Dr. Anubhav Sharma
Acid Fast Stain
Microbiology

Acid-Fast Stain: Principle, Reagents, Procedure and Result

Acid-Fast Stain- Principle, Reagents, Procedure and Result- Acid-fast staining is a differential staining technique used to identify mycobacteria. The mycobacteria are a group of bacteria that stain red with the acid-fast stain. The test helps diagnose infections with mycobacteria such as tuberculosis. What is an Acid-fast Stain? Acid-fast stain is a laboratory staining technique used to identify acid-fast bacteria, particularly Mycobacterium species such as Mycobacterium … Continue reading Acid-Fast Stain: Principle, Reagents, Procedure and Result

Dr. Anubhav Sharma1 Comment
Basic Fuchsin Solution/Stain
Microbiology

Basic Fuchsin Solution- Basic Fuchsin Stain

Basic Fuchsin Solution/Stain- Basic Fuchsin is a water-based mixture of basic fuchsine, known as rosaniline. It is a bacteriological staining agent and has a wide range of medical applications. When used as a stain, basic fuchsin produces a pink to red color with Gram-positive bacteria and a blue to purple color with Gram-negative bacteria. The Solution can be used to stain tissue samples for microscopy … Continue reading Basic Fuchsin Solution- Basic Fuchsin Stain

Dr. Anubhav Sharma1 Comment
Ziehl-Neelsen Stain
Microbiology

Ziehl-Neelsen Stain-Ziehl-Neelsen Staining Method

Ziehl-Neelsen Stain- The is a bacteriological staining technique to identify acid-fast organisms, such as Mycobacterium species. It is one of the most commonly used stains in microbiology and has been employed for over a century. The Ziehl-Neelsen stain is named after its inventors, German doctors Paul Ehrlich and Friedrich August Ziehl. What is the Ziehl-Neelsen Stain? The Ziehl-Neelsen stain is a specialized staining method used … Continue reading Ziehl-Neelsen Stain-Ziehl-Neelsen Staining Method

Dr. Anubhav Sharma1 Comment
Methyl Red Test Principle
Microbiology

Methyl Red Test Principle, Procedure and Result Interpretation?

Methyl Red Test is used to determine the ability of an organism to utilize glucose in the presence of oxygen. This test is performed by adding a small amount of methyl red to a culture of bacteria grown in various media, including mineral salts, meat extract, and peptone water. After incubation, the tubes are observed for a color change. A positive result will show a … Continue reading Methyl Red Test Principle, Procedure and Result Interpretation?

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pexels-photo-5841965.jpeg
Microbiology

What is the Difference between Pathogen and Parasite?

Difference between Pathogen and Parasite: We all know germs are everywhere on our hands, air, and surfaces. Some of these germs are harmless, while others can make us sick. So what’s the difference between a pathogen and a parasite? What is a Pathogen? Difference between Pathogen and Parasite: A pathogen is a microorganism that can cause disease. Pathogens can be bacteria, viruses, fungi, or protozoa. They can … Continue reading What is the Difference between Pathogen and Parasite?

Dr. Anubhav Sharma3 Comments
What is the Polymer of Fructose?
Biochemistry

What is the Polymer of Fructose?- What is Inulin?

What is the Polymer of Fructose? Fructose is a simple sugar that is found in many foods. It is a component of table sugar, fruits, and honey. In this article, we will take a closer look at the chemical structure of fructose and how it relates to other sugars. What is the Polymer of Fructose? The polymer of fructose is a type of sugar found … Continue reading What is the Polymer of Fructose?- What is Inulin?

Dr. Anubhav Sharma
Pyrogen Test
Microbiology

Pyrogen Test-How to Determine the Pyrogen Using Rabbits

Pyrogen Test- A pyrogen test is used to measure the presence of pyrogens in a sample. Pyrogens are substances that can cause fever, and the test is used to determine if a given substance is likely to cause this side effect. The test is performed by injecting a small amount of the substance into an animal, typically a rabbit, and then measuring the animal’s body … Continue reading Pyrogen Test-How to Determine the Pyrogen Using Rabbits

Dr. Anubhav Sharma2 Comments
open bottle and pills scattered around on table
Microbiology

Hold-Time Study for Pharmaceutical Products -Guidelines for Hold-Time Studies

Hold-Time Study for Pharmaceutical Products- A hold-time study aims to ensure that the medicinal product maintains its quality during storage and transport under the conditions specified on the label. The results of a hold-time study can be used to support the medicinal product’s stability and determine appropriate shelf life. There are several aspects to consider when designing a study to assess the stability of a … Continue reading Hold-Time Study for Pharmaceutical Products -Guidelines for Hold-Time Studies

Dr. Anubhav Sharma1 Comment
What is Plate Exposure Method Exposure Methods?
Microbiology

What is Plate Exposure Method! Exposure Methods?

Petri plate exposure method is used in microbiology and plant pathology to cultivate microorganisms or plant cells. The method involves the deposition of an inoculum (a suspension of microorganisms or plant cells) on a surface covered with a solid medium, such as agar or cell culture medium. The medium will then spread the inoculum over the surface, allowing it to grow. After some time – … Continue reading What is Plate Exposure Method! Exposure Methods?

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Sterility Test for Pharmaceutical Products
Microbiology

Sterility Test for Pharmaceutical Products, and How to perform it?

Sterility Test for Pharmaceutical Products- Sterility testing determines if a pharmaceutical product is free of viable microorganisms. The test is performed by incubating a product sample for some time and then checking for the growth of microorganisms. If no growth is observed, the product is considered sterile. Sterility testing is an important quality control measure for pharmaceutical products. It ensures that the products are free … Continue reading Sterility Test for Pharmaceutical Products, and How to perform it?

Dr. Anubhav Sharma2 Comments