Tag: Cell Culture

Cell Culture

Cell Culture Basics: Difference Between Cell lines

Cell Culture Basics: Difference Between Cells: Cell lines can be derived from different sources, such as tissues from animals or humans, or can be artificially created in the laboratory. Cell Culture Basics Cell culture (What is a Cell Culture) refers to the process of growing cells in the laboratory, either on a surface (such as a plastic dish) or in a liquid medium. Cell culture … Continue reading Cell Culture Basics: Difference Between Cell lines

Dr. Anubhav Sharma
laboratory equipment
Cell Culture

Types of Cell Culture Media- DMEM, IMDM, RPMI, and RPMI-H

Types of Cell Culture Media- Cell culture media is a complex mix of components used in the growth and maintenance of cultured cells. This article covers DMEM, IMDM, RPMI, and RPMI-H. You can also learn about other media types, including a serum-free alternative. Listed below are some differences between these media types. Table of Contents DMEM-Culture Media DMEM is cell culture media used for the … Continue reading Types of Cell Culture Media- DMEM, IMDM, RPMI, and RPMI-H

Dr. Anubhav Sharma
person holding test tubes
Cell Culture

Types, Limitations, and Applications of Cell Suspension Culture

Cell suspension culture is a technique that allows cells to grow in liquid medium. There are several different types of cell suspension cultures. In this article, we will look at their types, limitations, and applications. This technique is often used in research and biomedical applications. It is particularly useful for studying the development of new cancer drugs. Table of Contents What is Cell suspension culture? …

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Dr. Anubhav Sharma
person putting a drop on test tube
Cell Culture

Cell Culture Brief History, Importance, and Application

Cell Culture Brief History, Importance, and Application: Cell culture is a research method that allows single cells to function as independent units. The technique is used to study how drugs and viruses affect cells. It has been around for more than 100 years. In this article, we’ll talk about how cell culture works and its importance to science. Also, learn about some of the key …

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Dr. Anubhav Sharma
A scientist adding a stool sample to the cell culture medium, along with glass beads. Original image sourced from US Government department: Public Health Image Library, Centers for Disease Control and Prevention. Under US law this image is copyright free, please credit the government department whenever you can”.
Cell Culture

Cell Culture Media Components and Preparation- A Complete Guide

Cell Culture Media Components and Preparation- Cell culture media preparation involves a number of different steps. These include the selection of amino acids, buffering systems, proteins and Amino acid. Using the correct media preparation method will maximize the effectiveness of your experiments and ensure a high success rate. For more information, read the articles below. Cell Culture Media Components and Preparation Cell culture media is … Continue reading Cell Culture Media Components and Preparation- A Complete Guide

Dr. Anubhav Sharma
What is Embryonic Stem Cell Culture?
Cell Culture

What is Embryonic Stem Cell Culture? Complete Guide

What is Embryonic Stem Cell Culture? Embryonic stem cell culture is a process that can produce ES cells from embryos. This technique was first used by Martin Evans, who created a new way to cultivate mouse embryos in the uterus. Evans’ method is now used to isolate ES cells from mouse embryos. Table of Contents What is Embryonic stem cell culture? The study of embryonic … Continue reading What is Embryonic Stem Cell Culture? Complete Guide

Dr. Anubhav Sharma
Cell Culture

What does Adherent Cell Culture Mean? Comprehensive guide

What does Adherent Cell Culture Mean? The adherent cell culture is a form of cell culture in which cells grow in a flask until they cover the entire surface of the flask or until the medium becomes depleted of nutrients. The cells then need to be passaged or subculture to prevent the culture from dying. To do this, a protease is used to release cells …

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Dr. Anubhav Sharma
What is Primary and Secondary Cell Culture?
Cell Culture

What is Primary and Secondary Cell Culture? Complete Guide

What is Primary and Secondary Cell Culture? There are many different ways to obtain a cell culture. There are adherent and secondary cultures, Continuous cell lines, Single cell technology, and sub-culturing. Each has their own advantages and disadvantages, so you should be aware of the differences. Table of Contents Types of Cell Culture Adherent and secondary cell cultures Adherent and secondary cell cultures are two …

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Dr. Anubhav Sharma
Cell Culture

What is a Cell Culture? Application of Cell Culture?

What is a Cell Culture? Cell culture is a technique in which cells are grown under specific conditions to study their chromosomes and genes. Cells cultured under this technique may also be used in drug screening and development, the food industry, and medical research. However, it must be understood that a cell culture does not always work as expected. The loss of the cell’s ability …

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Dr. Anubhav Sharma
Methods to Estimate Confluency in Cell Culture?
Cell Culture

Methods to Estimate Confluency in Cell Culture? Complete Guide!

Methods to Estimate Confluency in Cell Culture? Cell culture monitoring is crucial in cell therapy applications. Cell viability assays can provide valuable information on the quality of the cells used in these applications. Live population tracking can also be very beneficial in stem cell cultures. The results of such analyses can be quantified with the use of image analysis algorithms. Moreover, live population tracking allows …

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Dr. Anubhav Sharma
How to Avoid Contamination During Cell Culture?
Cell Culture

How to Avoid Contamination During Cell Culture? Complete Guide!

How to Avoid Contamination During Cell Culture? Contamination in cell culture can cause problems. Contaminated cells are a waste and should not be used for any experiment. They are all hard to obtain and grow. The worst thing is that you might end up contaminating other people’s cells and results of experiments. Table of Content Techniques to Avoid Contamination in Cell Culture Pre-wetting techniques One …

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Dr. Anubhav Sharma
How to Maintain Sterility in Cell Culture Lab? Complete Guide
Cell Biology

How to Maintain Sterility in Cell Culture Lab? Complete Guide

How to Maintain Sterility in Cell Culture Lab? Cell culture labs are used to grow cells for research purposes. They provide a sterile environment for cell cultures so that researchers can study the effects of various chemicals on living organisms. Successful cell culture depends heavily on keeping the cells free from contamination by microorganisms such as bacterial, fungi, and viruses. Nonsterile supplies, media, and reagents, …

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Dr. Anubhav Sharma